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The Direct-zol™-96 RNA provides a streamlined method for the purification of up to 10 μg (per well) of high-quality RNA directly from samples in TRI Reagent?. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent? to the Zymo-Spin™ I-96 Plate, then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
Sample sources: Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent?, TRIzol?, RNAzol?, QIAzol?, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.