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Extremely sensitive. Highly specific. Exceptionally fast. Our SyGreen qPCR master mix utilizes the latest advances in polymerase technology and buffer chemistry to provide outstanding results on any DNA template including genomic, cDNA and viral sequences with the greatest efficiency. This all-in-one master mix requires little to no optimization. The 2x master mix uses an antibody-mediated hot start system that allows for a quick 2 minute activation step. This proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity.
The qPCRBIO SyGreen Mix utilizes a non-PCR inhibiting intercalating dye. Extremely low copy number targets can be detected specifically with the highest efficiency. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions on any real-time PCR cycler. Click on the Instrument Compatibility Guide below to find out which ROX level is compatible with your qPCR instrument.